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What can we make of gut microbiome research?

Hello, Recently, gut microbiome research and its application in diet and lifestyle advice has been called into question due to stool samples lacking in their capability to represent the actual intestinal tract (https://www.nature.com/articles/s41586-023-05989-7). I'm now wondering what to believe of all the "science-backed" messages out in the dietetics/nutrition world about "eating to improve your microbiome" and its associations with disease. How do we interpret this new evidence - do we have to throw out previous research? Thanks :)

FISH

Hello, my doubt is regarding fish, in terms of the evidence and your opinion, do you recommend it or not? I have my doubts. And because the consensus recommends it, if when compared, they come out badly, damaging health, my doubt is regarding that issue.

Body Composition, lifting and nutrition for the over 60's

I am now over 60 and lift heavy twice a week. What would be a reasonable body fat % for me to aspire to and how much daily protein should I be targetting in my diet?

protein powder absorption

Is it a waste to take too much protein powder at once because some of it won't get absorbed?

Animal Vs Plant (the norm subject)

Does the literature still show that an additional 30-40% of plant derived protein is required to equate a similar response from animal protein? Isolates and sources such as mucoprotein play a role? How does this increase play out when looking at the viability of plant protein in older populations that exhibit anabolic resistance? Is animal protein really still the gold standard? And required as a "high quality" source, especially when dealing with sarcopenic/ co-morbid individuals, who eat less/ move less etc?